Michael O'Brien is an American comedian who was a writer and short filmmaker on Saturday Night Live. He is the creator of the 2018 NBC comedy series A.P. Bio. O'Brien was born in Blissfield, Michigan to Connie and James O’Brien. He attended the University of Michigan, where he majored in film-video and was the founder and editor of a monthly comedy newspaper named The Anti-Daily.

O'Brien moved to Chicago and began taking improv and sketch classes. At iO Chicago he performed with a group called The Reckoning (2001–present). He trained at the Second City in Chicago, eventually joining the company's mainstage, where he performed for one year as a part of "America: All Better".